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anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488  (Proteintech)


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    Proteintech anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488
    Anti Resource Source Identifier Chromotek Gfp Booster Alexafluor 488 Proteintech Gb2af488, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+booster/CEL+Antibody/pm41205602-726-2-6
    Average 96 stars, based on 2373 article reviews
    anti resource source identifier chromotek gfp-booster alexafluor 488 proteintech gb2af488 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Transient pacing in pigs with complete heart block via myocardial injection of mRNA coding for the T-box transcription factor 18.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Sarcomeric alpha-actinin (Sigma-Aldrich; #A7811), GFP-Booster (Chromotek, #gba488-100), FLAG (Millipore Sigma; #F7425), GAPDH (BioRad; #MCA4739), Connexin-43 (Millipore Sigma; #C6219), Calnexin (Millipore Sigma; # SAB2501291) GFP (ThermoScientific; #A11122), CD45 (BioLegend; #202201), CD11b/c (BioLegend; #201801), CD8a (BioLegend; #201701), TBX18 (Invitrogen; #PA5-101921), Vimentin (Abcam; #ab24525), V5 tag (Abcam; #ab9116). ..

    Flow Cytometry:

    Article Title: Transient pacing in pigs with complete heart block via myocardial injection of mRNA coding for the T-box transcription factor 18.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Sarcomeric alpha-actinin (Sigma-Aldrich; #A7811), GFP-Booster (Chromotek, #gba488-100), FLAG (Millipore Sigma; #F7425), GAPDH (BioRad; #MCA4739), Connexin-43 (Millipore Sigma; #C6219), Calnexin (Millipore Sigma; # SAB2501291) GFP (ThermoScientific; #A11122), CD45 (BioLegend; #202201), CD11b/c (BioLegend; #201801), CD8a (BioLegend; #201701), TBX18 (Invitrogen; #PA5-101921), Vimentin (Abcam; #ab24525), V5 tag (Abcam; #ab9116). ..

    Magnetic Resonance Imaging:

    Article Title: Transient pacing in pigs with complete heart block via myocardial injection of mRNA coding for the T-box transcription factor 18.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Sarcomeric alpha-actinin (Sigma-Aldrich; #A7811), GFP-Booster (Chromotek, #gba488-100), FLAG (Millipore Sigma; #F7425), GAPDH (BioRad; #MCA4739), Connexin-43 (Millipore Sigma; #C6219), Calnexin (Millipore Sigma; # SAB2501291) GFP (ThermoScientific; #A11122), CD45 (BioLegend; #202201), CD11b/c (BioLegend; #201801), CD8a (BioLegend; #201701), TBX18 (Invitrogen; #PA5-101921), Vimentin (Abcam; #ab24525), V5 tag (Abcam; #ab9116). ..

    Incubation:

    Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation
    Article Snippet: .. 10 μm thick cryosections were blocked in PBS containing 2% bovine serum albumin, 5% normal donkey serum and 0.05% Tween-20 followed by incubation with the following reagents: GFP booster (catalog #gba488, Chromotek/ ProteinTech), anti-mCherry (catalog #orb11618, Biorbyt), and biotin-labeled anti-Epcam (clone G8.8, Biolegend). .. Sections were washed in PBS followed by incubation with Alexa Fluor Plus 555-labeled secondary antibody (catalog #A32816, Thermo Fisher Scientific) and Alexa Fluor 647-labeled streptavidin (catalog #S21374, Thermo Fisher Scientific).

    Article Title: COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans
    Article Snippet: .. Samples were incubated with primary antibodies overnight at 4°C at the indicated dilutions: mouse anti-FLAG (1:600, Sigma F1804), mouse anti-HA (1:600, BioLegend 16B12), GFP booster (1:400, Chromotek #gb2AF488), guinea pig anti-HTP-1 (1:300, generated by ABclonal Technology), rabbit anti-HIM-3 (1:300, generated by ABclonal Technology), rabbit anti-SYP-1 (1:300, generated by ABclonal Technology), rabbit anti-RAD-51 (1:1000, generated by ABclonal Technology), rabbit anti-OLLAS (1:500, Genscript A01658), and rabbit anti-MSH-5 pT1009 (1:300, obtained from Yumi Kim). .. Slides were washed with PBST and incubated with secondary antibodies at a 1:1000 dilution (Invitrogen Alexa 488, Alexa 594 or Alexa Fluor Plus 647) and DAPI (1:20) (Sangon Biotech E607303) for 2 h at room temperature.

    Article Title: COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans.
    Article Snippet: .. Samples were incubated with primary antibodies vernight at 4 ̊C at the indicated dilutions: mouse anti-FLAG 1:600, Sigma F1804), mouse anti-HA (1:600, BioLegend 6B12), GFP booster (1:400, Chromotek #gb2AF488), guinea ig anti-HTP-1 (1:300, generated by ABclonal Technology), abbit anti-HIM-3 (1:300, generated by ABclonal Technolgy), rabbit anti-SYP-1 (1:300, generated by ABclonal Techology), rabbit anti-RAD-51 (1:1000, generated by ABclonal echnology), rabbit anti-OLLAS (1:500, Genscript A01658), nd rabbit anti-MSH-5 pT1009 (1:300, obtained from Yumi im). .. Slides were washed with PBST and incubated with secndary antibodies at a 1:1000 dilution (Invitrogen Alexa 488, lexa 594 or Alexa Fluor Plus 647) and DAPI (1:20) (Sanon Biotech E607303) for 2 h at room temperature.

    Conjugation Assay:

    Article Title: Investigating the effect of bacteriophages on bacterial FtsZ localisation
    Article Snippet: The fixed cells were stained overnight at 4°C with anti-Prokaryotic Cell Division GTPase (FtsZ) antibody (Agrisera, Vännäs, Sweden) or rabbit anti- E. coli (strain K12) FtsI Polyclonal antibody if no fluorescent proteins were present and FtsZ or FtsI staining was needed, with diluted 1:100 in 0.05% saponin in PBS. .. This was followed by conjugation with secondary Donkey Anti-Rabbit IgG H&L (Alexa Fluor ® 488) diluted 1:500 in 0.05% saponin in PBS at room temperature for 45 min. For samples with fluorescent proteins, the fixed cells were stained with the following antibodies diluted in PBS/0.05% saponin for 60 min at room temperature: 5 μg/ml of GFP-booster (ChromoTek, Planegg, Germany), 5 μg/ml of RFP-booster (ChromoTek), and 5 μg/ml of phalloidin CF680R conjugate (Biotium, Fremont, CA, USA). .. After staining, the coverslips were washed, mounted on slides with DAPI-containing Fluoroshield Mounting Medium (Abcam, Cambridge, UK), and sealed.

    Staining:

    Article Title: Investigating the effect of bacteriophages on bacterial FtsZ localisation
    Article Snippet: The fixed cells were stained overnight at 4°C with anti-Prokaryotic Cell Division GTPase (FtsZ) antibody (Agrisera, Vännäs, Sweden) or rabbit anti- E. coli (strain K12) FtsI Polyclonal antibody if no fluorescent proteins were present and FtsZ or FtsI staining was needed, with diluted 1:100 in 0.05% saponin in PBS. .. This was followed by conjugation with secondary Donkey Anti-Rabbit IgG H&L (Alexa Fluor ® 488) diluted 1:500 in 0.05% saponin in PBS at room temperature for 45 min. For samples with fluorescent proteins, the fixed cells were stained with the following antibodies diluted in PBS/0.05% saponin for 60 min at room temperature: 5 μg/ml of GFP-booster (ChromoTek, Planegg, Germany), 5 μg/ml of RFP-booster (ChromoTek), and 5 μg/ml of phalloidin CF680R conjugate (Biotium, Fremont, CA, USA). .. After staining, the coverslips were washed, mounted on slides with DAPI-containing Fluoroshield Mounting Medium (Abcam, Cambridge, UK), and sealed.

    Generated:

    Article Title: COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans
    Article Snippet: .. Samples were incubated with primary antibodies overnight at 4°C at the indicated dilutions: mouse anti-FLAG (1:600, Sigma F1804), mouse anti-HA (1:600, BioLegend 16B12), GFP booster (1:400, Chromotek #gb2AF488), guinea pig anti-HTP-1 (1:300, generated by ABclonal Technology), rabbit anti-HIM-3 (1:300, generated by ABclonal Technology), rabbit anti-SYP-1 (1:300, generated by ABclonal Technology), rabbit anti-RAD-51 (1:1000, generated by ABclonal Technology), rabbit anti-OLLAS (1:500, Genscript A01658), and rabbit anti-MSH-5 pT1009 (1:300, obtained from Yumi Kim). .. Slides were washed with PBST and incubated with secondary antibodies at a 1:1000 dilution (Invitrogen Alexa 488, Alexa 594 or Alexa Fluor Plus 647) and DAPI (1:20) (Sangon Biotech E607303) for 2 h at room temperature.

    Article Title: COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans.
    Article Snippet: .. Samples were incubated with primary antibodies vernight at 4 ̊C at the indicated dilutions: mouse anti-FLAG 1:600, Sigma F1804), mouse anti-HA (1:600, BioLegend 6B12), GFP booster (1:400, Chromotek #gb2AF488), guinea ig anti-HTP-1 (1:300, generated by ABclonal Technology), abbit anti-HIM-3 (1:300, generated by ABclonal Technolgy), rabbit anti-SYP-1 (1:300, generated by ABclonal Techology), rabbit anti-RAD-51 (1:1000, generated by ABclonal echnology), rabbit anti-OLLAS (1:500, Genscript A01658), nd rabbit anti-MSH-5 pT1009 (1:300, obtained from Yumi im). .. Slides were washed with PBST and incubated with secndary antibodies at a 1:1000 dilution (Invitrogen Alexa 488, lexa 594 or Alexa Fluor Plus 647) and DAPI (1:20) (Sanon Biotech E607303) for 2 h at room temperature.

    other:

    Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation.
    Article Snippet: Sections were washed in PBS followed by incubation with Alexa Fluor Plus 555-labeled secondary antibody (catalog #A32816, Thermo Fisher Scientific) and Alexa Fluor 647-labeled streptavidin (catalog #S21374, Thermo Fisher Scientific).



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